Translational_Unit

Part:BBa_K1670005:Design

Designed by: Christoph Schilling   Group: iGEM15_Manchester-Graz   (2015-08-26)

EsaR repressor/activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 25


Design Notes

We use a D91G variant of the gene that shows higher sensitivity towards 3OC6-HSL [1]


Source

Synthesized gBlock according to the sequence information from gene sequencing from Erwinia stewartii GenBank: L32183.1 and [2]

Reference

1) Shong et al (2013) Directed Evolution of Quorum-Sensing Regulator EsaR for Increased Signal Sensitivity, ACS Chemical Biology 8 p.789-795
2) Shong et al (2013) Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression, ACS Chemical Biology 2 p.568-575